Regeneration of Escherichia coli Giant Protoplasts to Their Original Form
نویسندگان
چکیده
منابع مشابه
Acid-Soluble Nucleotides of Penicillin-Induced Protoplasts of Escherichia Coli **
The bactericidal action of penicillin is presently believed to result from alterations in nucleotide and peptide metabolism related to the structure of bacterial cell wall material. Park and Strominger have shown that in the penicillin-sensitive staphylococcus the drug causes the rapid accumulation of a group of uridine nucleotides."' These compounds contain a hexosamine, acetylmuramic acid, an...
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A preceding article (Lederberg, 1956a) was devoted to the evolution of protoplasts from growing cells of Escherichia coli treated with penicillin. Further studies have strengthened the correspondence of these protoplasts with the "large bodies" and "L forms" described for many other bacteria. This paper will give an account of these studies, and an interpretation of others, in support of the hy...
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A finely dispersed cell suspension was obtained from embryogenic calli of two genotypes of pearl millet (Pennisetum americanum L. Mil cereal). Protoplasts isolated from both cell suspensions divided and produced globular embryoids and calli. On an agar medium, the embryoids were induced to form albino shoots or albino plantlet- like structures
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Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...
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This article describes how to obtain isolated cells with no surrounding cell wall by enzymatic digestion of Ectocarpus filaments. The resultant protoplasts are totipotent and regenerate to produce individual algae under appropriate culture conditions. The yield of protoplasts and their capacity to regenerate are highly dependent on the Ectocarpus strain used, the stage of the life cycle, and th...
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ژورنال
عنوان ژورنال: Life
سال: 2019
ISSN: 2075-1729
DOI: 10.3390/life9010024